Barbara szymanska makuch biography of michael jackson
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Jacek Czekała, Alicja Jezierska, August Krzywosądzki
Calcium and magnesium content in treated waters and their total hardness
Key words: household water, hardness, calcium, magnesium.
Full text – pdf. DOI: 10.5601/jelem.2011.16.2.01
Household water is subject to special protection, as confirmed by the number of evaluated parameters, of which hardness and magnesium content deserve special attention. On the other hand, although calcium is not a limiting constituent although its concentration as well as the calcium compounds affect water hardness. Therefore, calcium is an element which is usually determined in raw water, after treatment as well as at the end-user. For hygienic reasons, particular attention is paid to magnesium concentration in water as well as quantitative relations between Mg and Ca.The aim of the study was to determine water hardness and the content of calcium and magnesium in treated wa
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To analyze the immunoglobulin repertoire of human IgM+ B cells and the CD5(+) and CD5(-) subsets, individual CD19(+)/ IgM+/CD5(+) or CD5(-) B cells were sorted and non-productive as well as productive VH gene rearrangements were amplified from genomic DNA and sequenced. In both subsets, the VH3 family was overrepresented largely as a result of preferential usage of a small number of specific individual family members. In the CD5(+) B cell subset, all other VH families were found at a frequency expected from random usage, whereas in the CD5(-) population, VH4 appeared to be overrepresented in the nonproductive repertoire, and also negatively selected since it was found significantly less often in the productive compared to the nonproductive repertoire; the VH1 family was significantly diminished in the productive rearrangements of CD5(-) B cells. 3-23/DP-47 was the most frequently used VH gene segment and was found significantly more often than expected from random usage in productive rearrangements of both CD5(+) and CD5(-) B cells. Evidence for selection based on the D segment and the JH gene usage was noted in CD5(+) B cells. No differences were found between the B cell subsets in CDR3 length, the number of N-nucleotides or evidence of exonuclease activity. Somatically hyper